Question: How Can I Tell the Difference Between a Male and Female Sugar Glider?
It is quite easy to tell the difference between a male and female sugar glider, especially once they have reached maturity. Females have a pouch on their bellies (which appears as about a one half inch slit), while males have a furry pendulous scrotum in front of the cloaca (the common opening of the reproductive,urinary, and intestinal tract). In young sugar gliders, the scrotum will not be as easy to see. Mature males also have a distinctive diamond shaped bald spot on top of their heads.
A good photo showing the scrotum of a male sugar glider (as well as the bald spot) can be found on this page of sugar glider anatomy.